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Tropical Journal of Pharmaceutical Research

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In-vitro anti-inflammatory activities of 3-methoxy quercetin isolated from Nigerian mistletoe parasitic on Garcinia kola Heckel, Clusiaceae

Edwin O. Omeje, Sylvester C. Nworu, Patience O. Osadebe, Lawrence Onugwu, Rakesh Maurya, Sunday N. Okafor, Peter Proksch

Abstract


Purpose: To evaluate the in vitro anti-oxidant and anti-inflammatory potential of the most potent and abundant metabolite, 3-methoxy quercetin (3-MQ), from extract fractions of mistletoe, Loranthus micranthus Linn (Loranthaceae) parasitic on Kola acuminata Schott & Endl, (Malvaceae), also known as Garcinia kola Heckel, (Clusiaceae).

Methods: Compounds isolated through a combination of chromatographic techniques were screened for in vitro antioxidant potential using the diphenyl picrazyl hydrazine (DPPH) radical-based model. Cell viability at 1–1000 μM 3-MQ in 24 h was evaluated by 3-(4, 5-dimethylthiazol-2-yl)-2, 5- diphenyltetrazolium bromide (MTT) test. Lipopolysaccharide (LPS), phorbol 12-myristate 13-acetate (n = 5) five (5) replicates.

Results: Ten (10) known compounds including 3-MQ (1) were isolated and characterized. 3-MQ exhibited highly significant (p < 0.05) antioxidant activity with 50 % inhibitory concentration (IC50) of 15.0 μM; concentrations ≤ 100 μM did not exert cytotoxic effect. 3-MQ, at 25 and 125 μM concentrations, significantly (p < 0.05) inhibited the production of TNF-α by 82 and 100 %, respectively, compared to controls.

Conclusion: The results demonstrate the potent anti-inflammatory activity of 3-MQ and suggests its use as a potential alternative therapy for inflammation and related diseases.

Keywords: Loranthus micranthus, Kola acuminata, Garcinia kola, Anti-inflammatory, Cytotoxicity, Chemiluminescence, Antioxidant, TNF-α




http://dx.doi.org/10.4314/tjpr.v16i5.13
AJOL African Journals Online