Anti-aging activities of Pyrus pyrifolia var culta plant callus extract
Abstract
Purpose: To investigate the anti-aging properties of callus extract from Pyrus pyrifolia var. culta with skin regeneration and lightening activities.
Methods: Callus extract of P. pyrifolia var. culta was produced on Murashige and Skoog (MS) medium supplemented with picloram, followed by extraction of the callus biomass in phosphate-buffered saline. The anti-oxidant activity of the extract was assessed using the DPPH free radical-scavenging assay. Inhibition of skin pigmentation by callus extract was evaluated by measuring the melanogenesis of melanoma cells. The skin-regenerating efficacy of the extract was evaluated in terms of its ability to promote procollagen synthesis and fibroblast cell proliferation.
Results: The callus extract, at 10 mg/mL, exhibited 78.7 % free radical-scavenging activity, equivalent to that exhibited by 500 μM ascorbic acid. The 1 mg/mL extract solution afforded a 1.4-fold greater reduction in melanocyte melanin than did a 1 mg/mL arbutin solution. Fibroblast cells treated with 5 mg/mL callus extract exhibited 127 % higher levels of proliferation and 2.0-fold higher levels of procollagen type I C-peptide synthesis, respectively, compared with the untreated controls.
Conclusion: These findings suggest that the callus extract of P. pyrifolia var. culta may be useful as a source of anti-aging cosmetic components with skin lightening and regenerating activities.
Keywords: P. pyrifolia var. culta, Anti-aging, Callus extract, Skin lightening, Anti-tyrosinase activity, Melanogenesis, Wound recovery, Procollagen type I C-peptide synthesis
Submission of a manuscript to this journal is a representation that the manuscript has not been published previously and is not under consideration for publication elsewhere.
All authors named in each manuscript would be required to sign a form (to be supplied by the Editor) so that they may retain their copyright in the article but to assign to us (the Publishers) and its licensees in perpetuity, in all forms, formats and media (whether known or created in the future) to (i) publish, reproduce, distribute, display and store the contribution, (ii) translate the contribution into other languages, create adaptations, reprints, include within collections and create summaries, extracts and/or abstracts of the contribution, (iii) create any other derivative works(s) based on the contribution, (iv) to exploit all subsidiary rights in the contribution, (v) the inclusion of electronic links from the contribution to third party material where-ever it may be located, and (vi) license any thrid party to do any or all of the above.