Extraction and purification of formonometin from Trifolium pratense L: Physicochemical properties of its complex with lecithin
Purpose: To isolate formononetin from Trifolium pratense L. (red clover), prepare its complex with lecithin foe enhanced solubility and evaluate its physicochemical properties.
Methods: Formononetin was extracted from red clover. The complex of formononetin with lecithin was prepared by solvent method in a ratio of 2.5:1.0 (lecithin : formononetin). The physicochemical properties of the complex were investigated by ultraviolet-visible spectrometry (UV/Vis), Fourier transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM), differential scanning calorimetry (DSC) and x-ray diffractometry (XRD).
Results: UV data showed that there was no significant difference (p < 0.05) between the peak intensity of the physical mixture and the complex, while FT-IR analysis indicated interaction between formononetin and lecithin. SEM showed that formononetin was successfully integrated in the structure of lecithin. DSC thermograms of the complex mainly demonstrated that the presence of lecithin caused the disappearing of characteristic endothermal peaks of formononetin, while x-diffractograms indicate that the crystalline peak of formononetin was absent in the complex.
Conclusion: The complex formed is held together by non-covalent-bonds and thus demonstrates new physical and chemical characteristics.
Keywords: Formononetin, Lecithin, Complex, Physicochemical characteristics
Submission of a manuscript to this journal is a representation that the manuscript has not been published previously and is not under consideration for publication elsewhere.
All authors named in each manuscript would be required to sign a form (to be supplied by the Editor) so that they may retain their copyright in the article but to assign to us (the Publishers) and its licensees in perpetuity, in all forms, formats and media (whether known or created in the future) to (i) publish, reproduce, distribute, display and store the contribution, (ii) translate the contribution into other languages, create adaptations, reprints, include within collections and create summaries, extracts and/or abstracts of the contribution, (iii) create any other derivative works(s) based on the contribution, (iv) to exploit all subsidiary rights in the contribution, (v) the inclusion of electronic links from the contribution to third party material where-ever it may be located, and (vi) license any thrid party to do any or all of the above.