Caulis Lonicerae Japonicae extract shows protective effect on osteoporosis in rats
Purpose: To investigate the effect of Caulis lonicerae Japonicae extract (CLJE) on ovariectomyinduced osteoporosis in rats.
Methods: Female Sprague-Dawley rats were randomly assigned to a control group (normal rats) and five ovariectomy (OVX) subgroups: OVX with vehicle (OVX), OVX with 17ß-estradiol (E2, 25μg/kg/day), and OVX with CLJE doses (150, 300, and 600 mg/kg/day). Daily oral administration of E2 or CLJE started 4 weeks after OVX and lasted for 16 weeks. The bone mineral density (BMD) of L4 vertebrae and right femurs was determined. The length of each femur was measured with a micrometer, and the center of the diaphysis was determined. Three representative L4 vertebrae were selected to evaluate trabecular microarchitecture. Serum alkaline phosphatase (ALP), urinary calcium (U-Ca), urinary phosphorus (U-P), urinary creatinine (Cr) and osteocalcin (OC) levels were measured using a diagnostic reagent kit.
Result: The results show that a high-dose of CLJE (600 mg/kg) significantly inhibited bone mineral density (BMD) reduction of L4 vertebrae (0.24 ± 0.02, p < 0.05) and femur (0.24 ± 0.03, p < 0.05) caused by OVX, and prevented the deterioration of trabecular microarchitecture (p < 0.05). High-dose CLJE also improved morphometric parameters, viz, trabecular number (Tb-N) (4.6 ± 0.3, p < 0.05), trabecular thickness (Tb-Th, 0.084 ± 0.012, p < 0.05) and trabecular separation (Tb-Sp, 0.14 ± 0.02, p < 0.05) in L4 vertebrae significantly.
Conclusion: The results indicate that CLJE prevents OVX-induced osteoporosis in rats. Thus, CLJE is a potential natural alternative for the treatment of postmenopausal osteoporosis in future.
Keywords: Caulis Lonicerae Japonicae, Post-menopausal osteoporosis, Ovariectomy, Bone mineral density, Trabecular microarchitecture, Diaphysis
Submission of a manuscript to this journal is a representation that the manuscript has not been published previously and is not under consideration for publication elsewhere.
All authors named in each manuscript would be required to sign a form (to be supplied by the Editor) so that they may retain their copyright in the article but to assign to us (the Publishers) and its licensees in perpetuity, in all forms, formats and media (whether known or created in the future) to (i) publish, reproduce, distribute, display and store the contribution, (ii) translate the contribution into other languages, create adaptations, reprints, include within collections and create summaries, extracts and/or abstracts of the contribution, (iii) create any other derivative works(s) based on the contribution, (iv) to exploit all subsidiary rights in the contribution, (v) the inclusion of electronic links from the contribution to third party material where-ever it may be located, and (vi) license any thrid party to do any or all of the above.