Effect of Yushen zhuyun decoction on rats with diminished ovarian reserve induced by tripterygium glycosides
Purpose: To investigate the effect of Yushen zhuyun decoction (YSZYF) on rats with diminished ovarian reserve (DOR).
Methods: High-performance liquid chromatography (HPLC) was used to determine the major phytochemical constituents of YSZYF. Rats with DOR (DOR rats) were prepared by administration of tripterygium glycosides (TWP) orally (50 mg/kg) for 15 days. Thereafter, DOR rats were treated orally with YSZYF (300, 600 and 900 mg/kg). After 15 days’ treatment, ovary index was calculated and blood was obtained to determine serum levels of follicle-stimulating hormone (FSH), estradiol (E2), progesterone (P), testosterone (T), inhibin (INH) and anti-mullerian hormone (AMH) by radioimmunoassay and enzyme-linked immunosorbent assay (ELISA). In addition, the ovary was subjected to histopathological examinations.
Results: Phytochemical investigation indicated that the major constituents of YSZYF are acteoside, loganin, lcariin and echinacoside. Compared to the control rats, YSZYF treatment enhanced the ovary index of DOR rat (p < 0.05); furthermore, YSZYF treatment also enhanced the number of follicles and corpus luteum, as well as alleviated inflammatory reaction in ovary tissues. Additionally, the serum levels of FSH and T were elevated by treatment of YSZYF (p < 0.01), whereas E2, INHB and AMH concentrations decreased (p < 0.01), compared to control rats.
Conclusion: The findings indicate that YSZYF improves ovarian reserve of DOR rats, and thus has a potential for treating infertility.
Keywords: Yushen zhuyun decoction, Diminished ovarian reserve, Infertility, Acteoside, Loganin, Lcariin, Echinacoside