Pharmacokinetics and tissue distribution of N-3- methoxybenzyl-palmitamide in rat: A macamide derived from Lepidium meyenii
Purpose: To study the pharmacokinetics and tissue distribution of N-3-methoxybenzyl-palmitamide (MPM) derived from Lepidium meyenii (Maca)
Methods: MPM and N-benzylpalmitamide (BPM, as the internal standard, IS) were prepared by one-pot synthesis method and characterized. For the analysis of MPM in rat plasma and tissue samples, a rapid ultra-performance liquid chromatography coupled to tandem mass spectrometry (UPLC-MS/MS) method was developed and validated by optimizing sample preparation conditions and UPLC conditions. Finally, the pharmacokinetics and biodistribution of MPM after oral administration in rats were studied.
Results: The lower limit of quantification (LLOQ) and limit of detection (LOD) of the UPLC-MS/MS method were 1.2 and 5.0 ng/mL, respectively. Good linear relationship of calibration curve (r > 0.9951) was achieved over the range of 5 – 5000 ng/mL. In pharmacokinetics, plasma concentration-time curve of MPM showed double peaks. The highest distribution of MPM after absorption was in the stomach, followed by lung. The absorption and eliminate rate of MPM were slow in rats. In fact, MPM displayed a lung targeting property.
Conclusion: The developed UPLC-MS/MS method is suitable for plasma and tissue distribution studies of MPM in rats. The present study can provide guidance for the further development and utilization of Maca tuber.
Keywords: Macamide, Maca tuber, Lepidium meyenii, Pharmacokinetics, Tissue distribution, UPLCMS/MS
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