PROMOTING ACCESS TO AFRICAN RESEARCH

Tropical Journal of Pharmaceutical Research

Log in or Register to get access to full text downloads.

Remember me or Register



Prophylactic effects of triptolide on colon cancer development in azoxymethane/dextran sulfate sodiuminduced mouse model

Yizhou Zhou, Li Sun, Lei Cui, Yu Feng

Abstract


Purpose: To investigate effects of triptolide on colon cancer cell growth and its capacity to prevent tumor development in an azoxymethane (AOM)/dextran sulfate sodium (DSS) mouse model of colon cancer.
Methods: HCT116 cell viability and migration potential were assessed. Control and AOM/DSS-treated mice (with and without triptolide) were analyzed for tumor development. The animals were divided into five groups (n = 5). Normal control group was given saline, animals in the untreated control group received AOM and DSS while animals in the treatment groups received 10, 50 and 100 mg/kg doses of triptolide intraperitoneally alternately for 2 months after AOM and DSS injection.
Results: Triptolide enhanced nuclear material condensation, significantly (p < 0.05) increased the levels of cleaved poly (ADP-ribose) polymerase, reduced the levels of pro-caspase-3 and pro-caspase-8 in HCT116 cells. Triptolide also significantly (p < 0.05) decreased the expression of pIκBα, activated peroxisome proliferator-activated receptor γ, and markedly reduced the activity of both metalloproteinase-2 and metalloproteinase-9. Treatment of AOM/DSS mice with triptolide significantly reduced adenocarcinoma multiplicity compared to the control group.
Conclusion: Triptolide administration suppresses growth of HCT116 cells and colon cancer development in mice by inhibiting inflammatory responses. Therefore, triptolide has potentials to be developed for colon cancer therapy.

Keywords: Cell viability, Condensation, Metalloproteinase, Apoptotic, Triptolide, Adenocarcinoma, Colon cancer




http://dx.doi.org/10.4314/tjpr.v16i11.13
AJOL African Journals Online