Detection of efflux pump activity among clinical isolates of Staphylococcus and Micrococcus species

  • Adebowale O Adeluola
  • Kolawole S Oyedeji
  • Udoma E Mendie
  • James R Johnson
  • John R Porter
Keywords: [1-(3-(Trifluoromethyl)benzyl]-piperazine, Efflux pump activity, Oxacillin resistant S. aureus, Multidrug resistant, Carbapenemase

Abstract

Purpose: To detect efflux pump activity (EPA) and screening a suspected efflux pump inhibitor (EPI) [1- (3-(trifluoromethyl)benzyl]-piperazine (TFMBP)], which could help in reducing multi-drug resistance (MDR).
Methods: Eighteen isolates, viz, 14 S. aureus, 2 S. lentus, 1 S. xylosus and 1 Micrococcus species from various hospital infections of admitted patients were screened for antibiotics susceptibility to 11 classes of antibiotics including oxacillin and β-lactamase production. Efflux pump activity (EPA) was determined by minimum inhibitory concentration (MIC) technique in the presence and absence of TFMBP, the isolates were also screened for MDR genes.
Results: All the isolates were resistant to ampicillin (10 μg) and penicillin (10 μg), but sensitive to bacitracin (10 μg). Majority of the isolates were MDR 12/18 (66.7 %), 10 (55.6 %) were inducible β- lactamase producers and 3 (16.7 %) were intrinsic β-lactamase producers. Seven (38.9 %) were resistant to oxacillin and also produced carbapenemase enzyme. Eight (66.7 %) of the 12 MDR isolates gave evidence of EPA with TFMBP. However, no MDR genes were detected.
Conclusion: Staphylococcus and Micrococcus species exhibit EPA in antibiotic resistance while a suitable EPI such as TFMBP when combined with specific antibiotics could help combat this menace.

Keywords: [1-(3-(Trifluoromethyl)benzyl]-piperazine, Efflux pump activity, Oxacillin resistant S. aureus, Multidrug resistant, Carbapenemase

Published
2018-01-03
Section
Articles

Journal Identifiers


eISSN: 1596-9827
print ISSN: 1596-5996