PROMOTING ACCESS TO AFRICAN RESEARCH

Tropical Journal of Pharmaceutical Research

Log in or Register to get access to full text downloads.

Remember me or Register



In vitro cytotoxicity of biosynthesized titanium dioxide nanoparticles in human prostate cancer cell lines

Feiping He, Weixing Yu, Xiaosong Fan, Baiye Jin

Abstract


Purpose: To establish a green method for production of titanium dioxide (TiO2) nanoparticles (NPs) using Cinnamomum tamala (C. tamala) leaf extract, and examine the in vitro cytotoxicity of the product in a human prostate cancer (D145) cell line. Methods: TiO2 NPs were synthesized by mixing 20 mL of C. tamala leaf extract with 0.1 M titanium dioxide (Ti(OH)2) (80 mL) in aqueous solution with stirring for 2 h at room temperature. The TiO2 NPs were characterized using x-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR), x-ray photoelectron spectroscopy, dynamic light scattering (DLS), transmission electron microscopy (TEM), selected-area electron diffraction, and energy dispersive x-ray spectroscopy. The in vitro cytotoxicity against D145 cells was determined using a 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide assay. Results: TEM and DLS analyses showed that the NPs were irregularly shaped, with an average particle size of 23 nm. The FT-IR spectrum of C. tamala leaf extract showed that the biomolecules were potentially involved in reduction processes. The negative zeta potential of -14 mV indicated that the NPs were stable and discrete while their crystalline nature was confirmed by XRD. Cytotoxicity analysis showed that the TiO2 NPs exhibit a dose-dependent toxic effect on D145 cells. Conclusion: A facile and less expensive approach for the production of TiO2 NPs using C. tamala leaf extract has been developed. The TiO2 NPs showed dose-dependent cytotoxicity towards D145 cells.

Keywords: Anticancer activity, Cinnamomum tamala, Green synthesis, Prostate cancer, TiO2 nanoparticles




AJOL African Journals Online