Effect of electrolyzed reduced water on malondialdehyde levels and neutrophil cells in aggressive periodontitis
Purpose: To evaluate the effects of electrolyzed reduced water (ERW) on malondialdehyde (MDA) levels and neutrophil cells in Wistar rats suffering from aggressive periodontitis.
Methods: Wistar rats (Rattus norvegicus) were infected with A. actinomycetemcomitans before being divided into a control group and a treatment group. The control group was treated orally with distilled water (pH 7.0), while the treatment group was administered with ERW (pH 9.8, 20 ml/day) for three successive days. Gingival tissue taken post-treatment was examined to determine the number of neutrophils and MDA level. Hematoxylin eosin staining was performed to identify neutrophil cells while MDA levels were determined by colorimetric assay.
Results: The number of neutrophils in the control group (30.04 ± 15.30) was higher than that of the treatment group (7.76 ± 2.52), while the MDA levels of the control and treatment groups were 4.58 ± 0.66 and 4.30 ± 0.81, respectively (p < 0.05).
Conclusion: ERW demonstrates the ability to reduce neutrophil cells without altering MDA levels in Wistar rats suffering from aggressive periodontitis. This is an indication that ERW was an effective material to manage aggressive periodontitis.
Keywords: Periodontitis, Electrolyzed reduced water, Malondialdehyde, Neutrophil cells
Submission of a manuscript to this journal is a representation that the manuscript has not been published previously and is not under consideration for publication elsewhere.
All authors named in each manuscript would be required to sign a form (to be supplied by the Editor) so that they may retain their copyright in the article but to assign to us (the Publishers) and its licensees in perpetuity, in all forms, formats and media (whether known or created in the future) to (i) publish, reproduce, distribute, display and store the contribution, (ii) translate the contribution into other languages, create adaptations, reprints, include within collections and create summaries, extracts and/or abstracts of the contribution, (iii) create any other derivative works(s) based on the contribution, (iv) to exploit all subsidiary rights in the contribution, (v) the inclusion of electronic links from the contribution to third party material where-ever it may be located, and (vi) license any thrid party to do any or all of the above.