Assessment of activity and mechanism of action of β-Dglucan against dengue virus
Purpose: To assess the antiviral efficiency of β-D-glucan (BDG) on human liver cell line (WRL68) infected with dengue virus (DENV).
Methods: Cytotoxic activity was assessed via 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Solid-phase virus binding assay was used to determine the presence of a chemical affinity between dengue virus type 2 (DENV-2) and BDG. Plaque formation assay was performed to measure the suppression of DENV-2.
Results: Plaque formation assay results revealed that the inhibition of DENV infection by BDG was effective at 400 μg/mL which occurred by inhibiting virus replication. BDG inhibited DENV replication and produced minimal toxicity on WRL68 cells at 600 μg/mL in a concentration-dependent manner. Treatment of DENV-2 with the highest concentration of the BDG resulted in 60, 55, and 50 % viability at 24, 48, and 72 h, respectively. Plaque formation and binding efficiency data confirmed that BDG protected the WRL68 cells against DENV-2.
Conclusion: The results indicate that in infected cells, β-D-glucan was found to be potent in inhibiting replication of the dengue virus.
Keywords: Dengue, β-D-glucan, Polysaccharide, Antiviral, Plaque formation, Binding efficiency