Root tuber extract of Polygooum cillinerve (Nakai) Ohwi exerts antimicrobial and immunomodulatory activities
Purpose: To investigate the antibacterial and immunomodulatory activities of the root tuber extract of Polygonum ciliinerve (Nakai) ohwi, a traditional Chinese medicine.
Methods: Minimum inhibitory concentrations (MICs) of root tuber extract of Polygonum ciliinerve (Nakai) ohwi (rPC) and the synergistic effects between rPC and antibiotics were evaluated by broth dilution. In vitro adhesion and invasion assays were used to determine the effect of rPC on bacterial adhesion and invasion. Furthermore, the immunomodulatory effects of rPC were assessed by western blot.
Results: rPC treatment inhibited the growth of Staphylococcus aureus (MIC = 12 mg/ml) and Escherichia coli (MIC = 64 mg/mL). rPC also showed synergistic effects with penicillin (fractional inhibitory concentration, FIC = 0.45), vancomycin (FIC = 0.333), moxifloxacin (FIC = 0.25), and levofloxacin (FIC = 0.356). The adhesion and invasion of bacteria were also suppressed by rPC treatment. Moreover, rPC exhibited an immunomodulatory effect during bacterial infection.
Conclusion: rPC shows antibacterial and immunomodulatory activites when assessed by multiple methods, and therefore is a potential therapeutic alternative agent for the treatment of bacterial infections.
Keywords: Polygonum ciliinerve root tuber, Synergistic effect, Immunomodulatory, Antibacterial
Submission of a manuscript to this journal is a representation that the manuscript has not been published previously and is not under consideration for publication elsewhere.
All authors named in each manuscript would be required to sign a form (to be supplied by the Editor) so that they may retain their copyright in the article but to assign to us (the Publishers) and its licensees in perpetuity, in all forms, formats and media (whether known or created in the future) to (i) publish, reproduce, distribute, display and store the contribution, (ii) translate the contribution into other languages, create adaptations, reprints, include within collections and create summaries, extracts and/or abstracts of the contribution, (iii) create any other derivative works(s) based on the contribution, (iv) to exploit all subsidiary rights in the contribution, (v) the inclusion of electronic links from the contribution to third party material where-ever it may be located, and (vi) license any thrid party to do any or all of the above.