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Evaluation of cytotoxic and wound healing effect of DMEM extracts of Turkish propolis in MDA-MB-231 cell lines

Meltem Uçar
Orhan Değer


Purpose: To investigate the effect of Dulbecco's Modified Eagle Medium (DMEM) extract of Turkish propolis on proliferation, cytotoxicity and lateral motility in MDA-MB-231 cells.

Methods: The antiproliferative activity of DMEM extracts of propolis was determined colorimetrically in MDA-MB-231 cells using 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) assay. Cell toxicity and wound healing effects of the prpolis extracts were determined with trypan blue exclusion assay and wound-healing assay, respectively.

Results: The cell number of MDA-MB-231 cells were decreased by the extracts at all concentrations for 72 h. The highest antiproliferative activity of the extract was demonstrated at 10 mg/mL for 24 - 72 h. Moreover, 5 and 0.31 mg/mL of the propolis extract showed significant antiproliferative activity at 72 h of incubation. The extract showed cytotoxic effect to MDA-MB-231 cells at 10 mg/mL. The extract (at a dose of 2.5 mg/mL) during 24 - 72 h did not produce any effect on lateral motility in MDA-MB-231 cells in the wound healing assay.

Conclusion: These results indicate that the DMEM extract of propolis exerts antiproliferative and cytotoxic effects on MDA-MB-231 cells at different concentrations.

Keywords: Propolis, Proliferation, Cytotoxicity, Motility, Breast cancer