Tropical Journal of Pharmaceutical Research

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In vitro bone sialoprotein-I expression in combined gingival stromal cells and platelet rich fibrin during osteogenic differentiation

Alexander Patera Nugraha, Ida Bagus Narmada, Diah Savitri Ernawati, Aristika Dinaryanti, Eryk Hendrianto, Igo Syaiful Ihsan, Wibi Riawan, Fedik Abdul Rantam


Purpose: To analyze the expression of bone sialoprotein - I (BSP - I) after the addition of platelet rich fibrin (PRF) in gingival somatic cell (GSC) culture medium during osteogenic differentiation in vitro.

Methods: GSCs were extracted from healthy, 1-month-old, male Wistar rats (Rattus Novergicus), weighing 250 - 300 g, and which had been randomly selected (n=4). These cells were cultured for 14 days and passaged every 4 days. Five subcultures of GSCs were cultured in three plates (M24) (N = 54; n = 6) for 7, 14 and 21 days in three preconditioned culture media (group I: plain culture media; group II: preconditioned osteogenic culture media, and group III: preconditioned osteogenic culture media with platelet rich fibrin). The expression of BSP-I was immunocytochemically (ICC) examined with monoclonal antibodies. Homogeneity and normality tests (p > 0.05) were then performed followed by an analysis of variance (ANOVA, p < 0.05).

Results: The highest expression of BSP-I was found in group III (Day 21, 13.00 ± 2.000), while the lowest expression of BSP-I was found in group I (Day 7, 7.33 ± 1.155). There were significant differences between the groups (p = 0.000, p < 0.05).

Conclusion: PRF stimulates and significantly enhances the expression of BSP-I in GSC culture during osteogenic differentiation. Thus, PRF can be used to accelerate regeneration of alveolar bone defects.

Keywords: Alveolar bone, Bone Sialoprotein-I, Gingival Somatic cells, Osteogenic ability, Platelet rich fibrin
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