Evaluation of clinical effectiveness of paclitaxel and ursolic acid co-loaded liposomes as enhanced treatment for head and neck squamous cell carcinoma

  • Bi-Hua Lv
  • Wei Tan
  • Xuejun Shang
  • Ling Zhang
Keywords: Paclitaxel, Ursolic acid, Combination chemotherapy, Head-and-neck squamous cancer

Abstract

Purpose: To enhance the clinical effectiveness of paclitaxel (PTX) by co-delivery with ursolic acid (UA) for the treatment of head and neck cancer.

Methods: Co-loaded liposomes of PTX and UA (UA-PTX-LiP) were prepared by thin-film hydration method. Their size and loading efficiency were determined using dynamic light scattering (DLS) technique and high performance liquid chromatography (HPLC), respectively. The effectiveness of UAPTX-LiP against HSC-3 human head and neck cancer cell-lines was compared with that of PTX liposome (PTX-LiP) using systemic cell-based in vitro evaluation with MTT assay. Fluorescent microscopy was used for cell uptake studies.

Results: The size of the prepared UA-PTX-LiP was 126.5 ± 3.22 nm. The ratiometric system for PTX and UA as liposom es revealed significantly enhanced cytotoxicity, with comparatively lower IC50, whencompared to individual PTX-Lip. Fluorescent microscopy revealed the internalization ability of UA-PTXLiP by targeted delivery of PTX in HSC-3 human head and neck cancer cell-line.

Conclusion: These results show that UA-PTX-LiP successfully enhances the therapeutic potential and clinical outcomes of PTX in head-and-neck cancer, and also demonstrate the useful effect of combination of UA and PTX in chemotherapy.

Keywords: Paclitaxel, Ursolic acid, Combination chemotherapy, Head-and-neck squamous cancer

Author Biographies

Bi-Hua Lv
Department of Pharmacy, Zhongnan Hospital of Wuhan University, Wuhan, Hubein 430071
Wei Tan
Department of Radiology, Hospital of Wuhan University of Science and Technology, Wuhan, 430081
Xuejun Shang
Department of Andrology, Jinling Hospital Affiliated to Nanjing University School of Medicine, Nanjing 210002
Ling Zhang
Department of Environmental Hygiene and Occupational Medicine, School of Public Health, Hubei Province Key Laboratory of Occupational Hazard Identification and Control, Wuhan University of Science and Technology, Wuhan 430081, China
Published
2019-03-16
Section
Articles

Journal Identifiers


eISSN: 1596-9827
print ISSN: 1596-5996