Compound Bieshe Kang’ai inhibits proliferation and induces apoptosis in HCT116 human colorectal cancer cells

  • Sha Wan
  • Li Tan
  • Nan Lei
  • Yurong Shi
  • Man He
  • Meng Zhang
  • Lijuan Zhou
  • Liangwen Jin
  • Xianli Meng
  • Kui Yang
  • Haibo Xu
Keywords: Apoptosis, BAX, Bcl-2, Cancer, Caspase, Compound Bieshe Kang’ai, Chromatin condensation, Nuclear fragmentation

Abstract

Purpose: To study the effect of Compound Bieshe Kang’ai (CBK) on proliferation and apoptosis in colorectal cancer cells.

Methods: HCT116 colorectal cancer cells and FHs 74 Int intestinal cells were treated with CBK, followed by determination of cell proliferation with 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Caspase-9 and caspase-3 activities as well as protein expressions of Bcl-2 and BAX, and mRNA levels of caspase-9, caspase-3, Bcl-2 and BAX in HCT116 cells were evaluated, followed by examination of the morphological alterations of HCT116 cells with Hoechst 33342 staining.

Results: CBK suppressed proliferation of HCT116 cells in a concentration- and time-dependent pattern, without cytotoxicity to FHs 74 Int cells. CBK also elevated caspase-9 and caspase-3 activities, mitigated protein translation of Bcl-2 and augmented that of BAX. It also enhanced mRNA transcriptions of caspase-9, caspase-3 and BAX, but decreased that of Bcl-2 in HCT116 cells in a  concentrationdependent manner, as well as induced cancer cell shrinkage, nuclear fragmentation and chromatin condensation.

Conclusion: The findings highlight CBK as a promising therapeutic agent for colorectal cancers, by retarding proliferation and inducing apoptosis in cancer cells.

Keywords: Apoptosis, BAX, Bcl-2, Cancer, Caspase, Compound Bieshe Kang’ai, Chromatin condensation, Nuclear fragmentation

Author Biographies

Sha Wan
Department of Pharmacology, School of Pharmacy, Chengdu University of Traditional Chinese Medicine
Li Tan
Department of Pharmacology, School of Pharmacy, Chengdu University of Traditional Chinese Medicine
Nan Lei
Department of Pharmacology, School of Pharmacy, Chengdu University of Traditional Chinese Medicine
Yurong Shi
Department of Pharmacology, School of Pharmacy, Chengdu University of Traditional Chinese Medicine
Man He
Department of Pharmacology, School of Pharmacy, Chengdu University of Traditional Chinese Medicine
Meng Zhang
Department of Pharmacology, School of Pharmacy, Chengdu University of Traditional Chinese Medicine
Lijuan Zhou

Institute for Chinese Medical Research, Sichuan Academy of Chinese Medical Sciences, Chengdu, China

Liangwen Jin
Department of Pharmacology, School of Pharmacy, Chengdu University of Traditional Chinese Medicine
Xianli Meng
Department of Pharmacology, School of Pharmacy, Chengdu University of Traditional Chinese Medicine
Kui Yang
Department of Pharmacology, School of Pharmacy, Chengdu University of Traditional Chinese Medicine
Haibo Xu
Department of Pharmacology, School of Pharmacy, Chengdu University of Traditional Chinese Medicine
Published
2019-03-16
Section
Articles

Journal Identifiers


eISSN: 1596-9827
print ISSN: 1596-5996