Curcumin protects trabecular meshwork cells against hydrogen peroxide-induced oxidative stress and apoptosis via Nrf2-keap1 pathway

  • Yi Luo
  • Hongtao Ding
  • Dujun Li
  • Jihong Luo
Keywords: Curcumin, Oxidative stress, Apoptosis, Glaucoma, Nrf2-Keap1

Abstract

Purpose: To explore the effects and molecular mechanism of action of curcumin on trabecular meshwork cells (TMCs).

Methods: TMCs were isolated from pig eyes and NF-E2-related factor 2 (Nrf2) was knocked down by siRNA transfection. The effect of curcumin on intracellular reactive oxygen species (ROS) was measured by a ROS-specific dye. Annexin V-FITC/propidium iodide (PI) double labeling was applied to determine apoptosis. The expressions of apoptosis-associated proteins and Nrf2-Kelch-like ECHassociated protein 1 (Keap 1) pathway activation were assessed by western blotting, while target gene transcription was analyzed by quantitative real-time polymerase chain reaction (qRT-PCR).

Results: Curcumin reduced the levels of intracellular ROS and apoptosis induced by H2O2, upregulated B-cell lymphoma-2 (Bcl-2), downregulated Bcl2-associated X (Bax), and activated caspase-3 and nine other proteins (p < 0.05). Curcumin reduced the expressions of Nrf2, HO-1, and NQO1, and increased Keap1 in H2O2-induced TMCs (p < 0.05). Moreover, Nrf2 knockdown partly reversed the effect of curcumin on ROS and apoptosis in TMCs induced by H2O2 (p < 0.05).

Conclusion: Curcumin inhibited oxidative stress and apoptosis by Nrf2-Keap1 activation in TMCs. Curcumin is therefore a potential therapeutic agent for the management of glaucoma.

Keywords: Curcumin, Oxidative stress, Apoptosis, Glaucoma, Nrf2-Keap1

Author Biographies

Yi Luo
Department of Ophthalmology,Wuhan Forth Hospital
Hongtao Ding
Department of Internal Medicine, Wuhan No. 1 Hospital, Wuhan 430022
Dujun Li
Department of Ophthalmology, Hubei Provincial Hospital of Traditional Chinese Medicine, Hubei Provincial Academy of Traditional Chinese Medicine, Hubei Provincial University of Traditional Chinese Medicine, Hubei 430065, China
Jihong Luo
Department of Ophthalmology, Hubei Provincial Hospital of Traditional Chinese Medicine, Hubei Provincial Academy of Traditional Chinese Medicine, Hubei Provincial University of Traditional Chinese Medicine, Hubei 430065, China
Published
2019-03-16
Section
Articles

Journal Identifiers


eISSN: 1596-9827
print ISSN: 1596-5996