Anhuienoside C ameliorates atherosclerosis in rats via regulation of the NFκB/eNOS/NO signaling pathway
Purpose: To investigate the protective effect of anhuienoside C (AC) against high cholesterol dietinduced atherosclerosis in a rat model.
Methods: Atherosclerosis was induced in rats by administration of high fat diet for 8 weeks, and AC (20 and 40 mg/kg) was administered orally. The effect of AC was determined by assessing serum lipid profiles and mediators of inflammation, as well as oxidative stress parameters in aortic tissue using enzyme-linked immunosorbent assay (ELISA). Western blot assay and reverse transcription polymerase chain reaction (RT-PCR) were used for the evaluation of protein expressions.
Results: Serum levels of total cholesterol (TC), triglycerides (TGs), high density lipoprotein (HDL), low density lipoprotein (LDLP), and IL-1β, IL-18, TNF-α and NF-κB were significantly reduced in AC-treated group, relative to atherosclerotic rats (p < 0.01). Moreover, parameters of oxidative stress were attenuated in the aortic tissues of AC-treated group, when compared with atherosclerotic rats. There was significant increase in eNOS expression, and marked decrease in the expressions of MAPK and NF-kB protein in the aortic tissue homogenate of AC treated group, relative to atherosclerotic group (p <0.01). Treatment with AC attenuated the mRNA expressions of ICAM-1, VCAM-1 and MCP-1 in aortic tissue of the atherosclerotic rats.
Conclusion: These results reveal that AC prevents atherosclerosis in rats by modulating the NFκB/eNOS/NO signaling pathway, and thus, can thus potentially be developed as anti-atherosclerotic agent.
Keywords: Anhuienoside C, Atherosclerosis, Inflammation, Oxidative stress, Cholesterol
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