PSAT1 prompted cell proliferation and inhibited cell apoptosis in multiple myeloma through regulating PI3K/AKT pathway

  • Hongqing Zhu
  • Yejun Si
  • Yun Zhuang
  • Meng Li
  • Jianmin Ji
  • Ou Ji
  • Qun Shen
Keywords: Multiple myeloma, PSAT1, Cell proliferation, PI3K/AKT pathway

Abstract

Purpose: To identify the biological function of phosphoserine aminotransferase 1 (PSAT1) in regulating cell proliferation and apoptosis in multiple myeloma (MM).
Methods: The mRNA and protein levels of PSAT1 were determined using quantitative real-time polymerase chain reaction (PCR) and western blotting, respectively. Cell proliferation was measured using CCK-8 assay.
Results: PSAT1 mRNA and protein expression levels were significantly increased in MM cell lines when compared to control cells. Moreover,  downregulation of PSAT1 inhibited MM cell proliferation and induced cell apoptosis, whereas overexpression of PSAT1 promoted MM cell  proliferation and suppressed cell apoptosis. Further analysis demonstrated that the underlying mechanism was via regulation of PI3K/AKT pathway.
Conclusion: The results identified a novel role for PSAT1 in the progression of MM, which may provide a therapeutic and a new anticancer target for the therapy of MM.

Keywords: Multiple myeloma, PSAT1, Cell proliferation, PI3K/AKT pathway

Published
2020-05-14
Section
Articles

Journal Identifiers


eISSN: 1596-9827
print ISSN: 1596-5996