Ultra-performance liquid chromatography–tandem mass spectrometric determination of ramipril in human plasma

  • Sherif A. Abdel-Gawad
  • Safar M. Alqahtani
Keywords: Liquid chromatography–tandem mass, Ramipril, Stability, Biological fluids, Plasma

Abstract

Purpose: To develop a sensitive and accurate ultra-performance liquid chromatography–tandem mass spectrometric (UPLC-MS) method for quantification of ramipril in human plasma.
Methods: Ramipril was extracted from biological fluid using equal volumes of n-hexane and propanol (1:1, v/v), and then chromatographed in a suitable C18 column with methanol: 0.1 % HCOOH (4: 1, v/v) as mobile phase. Atorvastatin was used as an internal standard for the  chromatographic separation and quantification. The method was validated according to the United States Food and Drug Administration guidelines for standard indices.
Results: Ramipril was determined in the concentration range 0.05 and 1000 ng/mL the validation procedure exhibited a correlation coefficient of 0.9979 + 0.002 (p = 0.05). The studied drug was quantified with lower ceiling of 0.05 ng/mL, and showed an accuracy of 105.00 %.
Conclusion: A sensitive UPLC-MS analytical method has been successfully developed for the quantification of ramipril in human plasma. This method can be applied efficiently for the quantification of ramipril in bioavailability and pharmacokinetic studies.

Keywords: Liquid chromatography–tandem mass, Ramipril, Stability, Biological fluids, Plasma

Published
2020-05-15
Section
Articles

Journal Identifiers


eISSN: 1596-9827
print ISSN: 1596-5996