TRIM 16 gene expression regulates the growth and metastasis of human esophageal cancer

  • Yufang Chen
  • Ziyan Li
  • Jingyao Zeng
  • Zhiyi Xu
  • Meihua Wang
Keywords: Apoptosis, Cell invasion, Esophageal cancer, Gene silencing, Metastasis


Purpose: To investigate the effect of tripartite membrane protein  TRIM) 16 gene silencing on human esophageal cancer (KYSE-270) cell proliferation, invasion and metastasis.
Methods: Short interfering RNA (siRNA) TRIM 16 silencing fragment was transfected into KYSE-270 cells. Transfection efficiency was determined using real-time quantitative polymerase chain reaction (qRT-PCR). Cell proliferation, invasiveness and migration were measured by 3-(4,5-dimethylthiazol2-yl)- 2,5-diphenyl tetrazolium bromide (MTT) assay, Transwell invasion assay, and scratch test, respectively. Protein expressions of bax and bcl-2 were assayed using Western blotting.
Results: The gene expression of TRIM 16 was significantly upregulated in esophageal cancer cells, relative to normal human esophageal cells, but was downregulated after gene silencing. Moreover, the silencing of TRIM 16 gene led to significant reductions in KYSE-270 cell viability, migration and invasiveness, but significantly increased KYSE270 cell apoptosis (p < 0.05). The silencing of TRIM 16 gene also significantly upregulated bax protein expression, while downregulating the expression of bcl-2 protein (p < 0.05).
Conclusion: These results suggest that TRIM 16 gene silencing inhibits KYSE-270 cell proliferation, invasion and metastasis, and thus provide a basis for its development as a therapeutic approach for the management of esophageal cancer.

Keywords: Apoptosis, Cell invasion, Esophageal cancer, Gene silencing, Metastasis


Journal Identifiers

eISSN: 1596-9827
print ISSN: 1596-5996