Anticancer activity of okra raw polysaccharides extracts against human liver cancer cells
Abstract
Purpose: To examine raw polysaccharides extract of okra (Abelmoschus esculentus L.) as an anticancer agent against human liver cancer cells.
Methods: Okra raw polysaccharide extract (ORPE) was obtained by ethanol extraction from the raw fruit. Huh7it cells were grown in DMEM (Dulbecco's Modified Eagle Medium) and cultured for 24 h prior to treatment with the extract. The cell culture was divided into 3 groups, viz, negative control group (KN), positive control group (KP, treated with 10 μg/mL doxorubicin), and ORPE (P) group. ORPE group was divided into 5 subgroups based on the dose used for treatment, viz, 50 (P1), 100 (P2), 200 (P3), 400 (P4), and 600 µg/mL (P5). Huh7it cell proliferation was measured by MTT assay. while measurement of Huh7it cell apoptosis, necrosis, and cell cycle analysis were carried out using Annexin V FITC-PI antibody test and flow cytometry.
Results: ORPE significantly inhibited Huh7it cell proliferation and induced apoptosis. ORPE treatment with 600 µg/mL extract caused 5.82 % late cell apoptosis and 5.62 % early apoptosis. Cell cycle arrest occurred during G0/G1 phase.
Conclusion: ORPE is a potential anticancer agent against human liver cancer cells due to its ability to induce apoptosis of huh7it cells by promoting cell cycle arrest during G0/G1 phase.
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