Article Sidebar
Published:
Jul 7, 2021Keywords:
Article Details
Submission of a manuscript to this journal is a representation that the manuscript has not been published previously and is not under consideration for publication elsewhere.
All authors named in each manuscript would be required to sign a form (to be supplied by the Editor) so that they may retain their copyright in the article but to assign to us (the Publishers) and its licensees in perpetuity, in all forms, formats and media (whether known or created in the future) to (i) publish, reproduce, distribute, display and store the contribution, (ii) translate the contribution into other languages, create adaptations, reprints, include within collections and create summaries, extracts and/or abstracts of the contribution, (iii) create any other derivative works(s) based on the contribution, (iv) to exploit all subsidiary rights in the contribution, (v) the inclusion of electronic links from the contribution to third party material where-ever it may be located, and (vi) license any thrid party to do any or all of the above.
Main Article Content
Curcumin suppresses leukemia cell proliferation by downregulation of P13K/AKT/mTOR signalling pathway
Abstract
Purpose: To investigate the effect of curcumin ester on the proliferation of leukemia cell lines in vitro.
Methods: Changes in WEHI-3 and THP 1 cell viabilities were measured using Cell Counting Kit 8 (CCK 8). Analysis of cell cycle and determination of apoptosis were carried out using propidium iodide and Annexin V fluorescein isothiocyanate staining. Transmission electron microscopy was used for observing the presence of apoptotic features in cells.
Results: Treatment with curcumin ester for 72 h caused significant reduction in the proliferation of WEHI-3 and THP 1 cells. Curcumin ester, at a dose of 50 µM, decreased the proliferations of WEHI-3 and THP 1 cells to 28 and 32 %, respectively. On exposure to curcumin ester for 72 h, cell cycle in WEHI-3 cells was arrested in G1/G0 phase. Curcumin ester at doses of 25, 30 and 50 µM enhanced apoptosis in WEHI-3 cells to 46, 58 and 64 %, respectively. Curcumin ester suppressed the levels of phosphoinositide 3 kinase (PI3K), protein kinase B (AKT) and mechanistic target of rapamycin (mTOR) protein and mRNA in WEHI-3 cells. In curcumin ester-treated WEHI-3 cells, the presence of apop¬totic bodies increased significantly and concentration-dependently.
Conclusion: These results demonstrate that curcumin ester inhibits leukemia cell proliferation by inducing apoptosis and arresting cell cycle in G1/G0 phase, probably via suppression of PI3K, AKT and mTOR, and promotion of PTEN. Thus, curcumin ester has potentials for use in the development of an effective treatment strategy for leukemia.
