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<i>Brucea javanica</i> oil emulsion induces autophagy in glioma cells via lncRNA-p21/miR-17-5p/PTEN pathway
Abstract
Purpose: To investigate the possible effects of Brucea javanica oil emulsion in the progression of glioma, and elucidate the possible regulatory mechanism.
Methods: MTT assays were performed to assess the effects of BJOE on the viability of glioma cells, and flow cytometry (FCM) was conducted to evaluate its effect of the oil on cell apoptosis. Immunoblot assays were carried out to investigate its effects on glioma cell autophagy and LincRNA-p21/miR-17-5p/PTEN axis of glioma cells.
Results: Administration of BJOE (1, 2 and 4 mg/mL) increased the protein levels of LC3 II/ LC3 I and Beclin-1, but decreased the protein levels of p62 (p < 0.05). These changes were reversed by knockdown PTEN. Moreover, the expression of lncRNA-p21 and PTEN mRNA were significantly elevated, while the expression of miR-17-5p significantly decreased in BJOE-treated U251MG cells (p < 0.05).
Conclusion: BJOE may serve as a novel therapeutic drug for the treatment of glioma; The antitumor effect of BJOE is based on its ability to regulate lncRNA-p21/miR-17-5p/PTEN pathway.
