MFAP2 contributes to the proliferation and motility of lung cancer cells via Wnt/β-catenin pathway

  • Xiuyun He
  • Yunfeng Qiao
Keywords: Microfibrillar-associated protein 2 (MFAP2), Lung cancer, Viability, Motility, Wnt/β-catenin pathway

Abstract

Purpose: To measure microfibrillar-associated protein 2 (MFAP2) expression levels in lung cancer and identify its role in lung cancer.
Methods: Immunoblots and PCR were used to determine the expression of MFAP2 in lung cancer cell lines. The effects of MFAP2 on the viability and apoptosis of lung cancer cells were evaluated by CCK-8, colony formation, and flow cytometry (FCM) assays, while wound healing and Transwell assays were used to assess the effects of MFAP2 on the motility and epithelial-mesenchymal transition (EMT) of lung cancer cells. Immunoblot assays were also performed to determine the effect of MFAP2 on the Wnt/β-catenin axis in lung cancer cells.
Results: MFAP2 was highly expressed in lung cancer cells. Depletion of MFAP2 suppressed the viability and stimulated apoptosis in these cells (p < 0.01). In addition, knockdown of MFAP2 suppressed the motility and EMT of lung cancer cells (p < 0.01). MFAP2 mediated the Wnt/β-catenin axis and affected the viability and motility of lung cancer cells.
Conclusion: MFAP2 is a promising target for lung cancer treatment.

Published
2022-06-16
Section
Articles

Journal Identifiers


eISSN: 1596-9827
print ISSN: 1596-5996