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Published:
Aug 19, 2022DOI:
10.4314/tjpr.v21i7.6Keywords:
Article Details
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Main Article Content
MicroRNA-542 suppressed the proliferation of human glioma cells by targeting talin-2 (TLN2)
Abstract
Purpose: To investigate the effect of miR-542 in the development of human glioma.
Methods: The expressions of miR-542 and TLN2 in glioma cells and normal human astrocytes were determined using qRT-PCR, while MTT and colony formation assays were used to determine cell proliferation. Western blotting was used to determine protein expression.
Results: It was revealed that miR-542 was significantly downregulated in glioma cells. Overexpression of miR-542 inhibited the proliferation and clonogenicity of glioma cells via induction of apoptosis. The percentage of apoptotic U87 cells increased from 5.32 in control to 26.76 upon miR-542 overexpression. Moreover, TLN2 was identified as the functional regulatory target of miR542 in glioma. The expression of TLN2 was markedly upregulated in human glioma cells. However, overexpression of miR-542 suppressed TLN2 expression. Silencing of TLN2 mimicked the tumor-suppressive effects of miR-542 in glioma cells, but this effect was blocked by TLN2 over-expression.
Conclusion: These results suggest that miR-542 exerted glioma-suppressive effect, with TLN2 as its functional regulatory target.
Keywords: Glioma; Proliferation; Micro-RNA; Tumorigenesis; MiR-542; Apoptosis; Prognosis; talin-2; Oncogene
