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MiR-25 enhances the proliferation, invasion and migration of nasopharyngeal carcinoma cells through targeted regulation of RAGE expression

Yang Jing
Qingxia Zhao
Yujuan Wang
Pei Lin


Purpose: To determine the effect of microRNA-25 (miR-25) on the proliferation, invasion, and migration of nasopharyngeal carcinoma  cells, and the involvement of targeted regulation of late glycation end product receptor (RAGE) in the process.
Methods: Three groups of cells comprising; routinely cultured nasopharyngeal normal epithelial cell line NP69 without any treatment  (blank control group), untreated human nasopharyngeal carcinoma cell line 5-8F, also cultured routinely (nasopharyngeal carcinoma  group) and 5-8F human nasopharyngeal carcinoma cells infected with lentiviral vectors for miR-25 gene knockdown and cultured after  stable transduction (miR-25 knockdown group). Cell proliferation was assessed using CCK-8 assay, while Western blot assay and  quantitative polymerase chain reaction (qPCR) were used to measure protein and mRNA expression levels of relevant genes. Transwell  assay was utilized to evaluate cell migration and invasion.
Results: The miR-25 expression level was significantly increased in nasopharyngeal carcinoma group (p < 0.05). Cell proliferation,  migration, and invasion rates in miR-25 knockdown group were lower than those in nasopharyngeal carcinoma group (p < 0.05).  Apoptosis rate of cells and levels of apoptotic proteins were significantly elevated, whereas bcl-2 level was significantly reduced in miR-25  knockdown group when compared to nasopharyngeal carcinoma group (p < 0.05). Protein expression levels of RAGE and S100P was  significantly increased in nasopharyngeal carcinoma group, but significantly down-regulated in miR-25 knockdown group (p < 0.05).  Conclusion: Expression of miR-25 increases in nasopharyngeal carcinoma cells. Suppression of miR25 results in decreased viability of  nasopharyngeal carcinoma cells and inhibits cell proliferation, migration, and invasion, while enhancing apoptosis. The mechanism  underlying these effects was associated with modulation of protein expressions of RAGE and S100P. Targeted regulation of late glycation  end-product receptors hold promising potential as prospective biomarker for therapeutic interventions targeting specific cancers.

Journal Identifiers

eISSN: 1596-9827
print ISSN: 1596-5996