Article Sidebar
Published:
Apr 4, 2025Keywords:
Article Details
Submission of a manuscript to this journal is a representation that the manuscript has not been published previously and is not under consideration for publication elsewhere.
All authors named in each manuscript would be required to sign a form (to be supplied by the Editor) so that they may retain their copyright in the article but to assign to us (the Publishers) and its licensees in perpetuity, in all forms, formats and media (whether known or created in the future) to (i) publish, reproduce, distribute, display and store the contribution, (ii) translate the contribution into other languages, create adaptations, reprints, include within collections and create summaries, extracts and/or abstracts of the contribution, (iii) create any other derivative works(s) based on the contribution, (iv) to exploit all subsidiary rights in the contribution, (v) the inclusion of electronic links from the contribution to third party material where-ever it may be located, and (vi) license any thrid party to do any or all of the above.
Main Article Content
Physicochemical, GC-MS, antimicrobial, and antioxidant assays of Persea americana (Avocado) seed extract
Abstract
Purpose: To investigate the phytoconstituents, gas chromatography-mass spectrometry (GC-MS), antioxidant, and antimicrobial properties of Persea americana Mill. (Fam. Lauraceae) seeds extract and fractions. Methods: The plant seeds were air-dried, extracted with methanol, and fractionated using n-hexane, ethyl acetate, and methanol, respectively. Phytoconstituents of the extract and fractions and antioxidant activity were evaluated using GC-MS and 2,2-diphenyl-1-picrylhydrazyl (DPPH) at different concentrations (500, 250, 125, and 75 mg/mL). Antimicrobial activity evaluation against Staphylococcus aureus, Bacillus subtilis, Pseudomonas aeruginosa, Candida albicans, and Escherichia coli was carried out using the agar well-diffusion method with ciprofloxacin as positive control. Results: Qualitative analysis revealed the presence of alkaloids, anthocyanins, flavonoids, tannins, phenolics, carbohydrates, and terpenoids. Crude extract showed higher carbohydrate content (22.63 ± 0.73 mg/g) than fractions. Saponins were absent in extract and fractions. In vitro assays, including DPPH radical scavenging and total antioxidant capacity, demonstrated high oxidative activity of Persea americana seed extracts and fractions. The samples showed antimicrobial activities, with crude extract and n-hexane fraction having a minimum inhibitory concentration (MICs) of 31.25 mg/mL. Conclusion: The findings of this study demonstrate the presence of various phytoconstituents, as well as the antioxidant and antimicrobial activities of the extracts and fractions of Persea Americana seeds. There would be need for studies to evaluate the toxicologic profile of the bioactive compounds in order to ascertain safety.
