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Tropical Journal of Pharmaceutical Research

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Anti-Cancer Properties of Diethylether Extract of Wood from Sukun (Artocarpus altilis) in Human Breast Cancer (T47D) Cells

ET Arung, BD Wicaksono, YA Handoko, IW Kusuma, D Yulia, F Sandra

Abstract


Purpose: To evaluate the anti-cancer properties of the diethylether extract of Sukun (Artocarpus altilis) wood.
Methods: The extract was tested in human T47D breast cancer cells and examined for its effect on cell viability, nuclear morphology and sub-G1 formation. Cell viability was determined by microculture tetrazolium technique (MTT), nuclear morphology investigated using 4’-6-diamidino-2-phenylindole (DAPI) staining technique, and cell cycle progression monitored by sub-G1 apoptosis assay using flow cytometry.
Results: The results showed decreasing cell viability in a concentration-dependent manner. Altered cell morphology after treatment with the extract demonstrated that cells experienced apoptosis. Cell cycle analysis indicated that the number of cells in sub-G1 phase rose with increasing concentrations of the extract.
Conclusion: The data demonstrate that Sukun wood extract induced apoptosis and sub-G1 phase formation in breast cancer (T47D) cells, and therefore, has a potential as an anti-cancer agent.



http://dx.doi.org/10.4314/tjpr.v8i4.45223
AJOL African Journals Online