Tropical Journal of Pharmaceutical Research

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Isolation and morphological characterization of antibiotic producing actinomycetes

KN Basavaraj, S Chandrashekhara, AM Shamarez, PS Goudanavar, FV Manvi


Purpose: To isolate and characterize antibiotic producing actinomycetes from soil samples in Belgaum, Karnataka, India. Methods: Crowded plate technique was used for the isolation of actinomycetes in media such as
soybean – casein digest medium and actinomycetes isolation agar. The morphological and cultural characterization of one of the selected strains, designated A-4, was performed as per International Streptomycete Project (ISP). Results: Morphological and cultural studies showed that A-4 belonged to the Actinomycete genus. The morphological and cultural characteristics of the A-4 mutant showed cellular and aerial growth as well as soluble pigment formation in various ISP media. Conclusion: Findings from this investigation revealed that the selected strain, A-4, is an actinomycete.

Keywords: Actinomycete, Antibiotic, MIC, Crowded plate technique, Morphology
AJOL African Journals Online