Determination of Venlafaxine and Modafinil in Individual Tablet Dosage Forms using Single RP-HPLC Method
Purpose: To develop a simple and selective isocratic method for the determination of venlafaxine and modafinil in tablet dosage forms.
Methods: The compounds were analyzed on Waters symmetry C18 column (4.6 mm x 250 mm i.d, 5ìm) using a mobile phase consisting of a mixture of ammonium acetate buffer (pH was adjusted to 4.0 with glacial acetic acid):10 % methanol in acetonitrile, in the ratio of 60:40. The flow rate was 1.0 ml/min and column effluents were monitored at 225 nm. The method was validated according to ICH guidelines.
Results: Venlafaxine and modafinil were eluted with retention times of 4.416 min and 6.443 min, respectively. The method was linear in the range of 1.0 - 50 ìg/ml for both venlafaxine and modafinil. The relative standard deviation (%RSD) was < 1 for both drugs while mean recovery values at different concentration levels were within limits. The performance of the method was not changed when small variations in the method were made.
Conclusion: The proposed method is accurate, reproducible and low-cost, and can be used for the routine analysis of the individual drugs in formulations.
Keywords: Venlafaxine, Modafinil, Isocratic method, Validation
Submission of a manuscript to this journal is a representation that the manuscript has not been published previously and is not under consideration for publication elsewhere.
All authors named in each manuscript would be required to sign a form (to be supplied by the Editor) so that they may retain their copyright in the article but to assign to us (the Publishers) and its licensees in perpetuity, in all forms, formats and media (whether known or created in the future) to (i) publish, reproduce, distribute, display and store the contribution, (ii) translate the contribution into other languages, create adaptations, reprints, include within collections and create summaries, extracts and/or abstracts of the contribution, (iii) create any other derivative works(s) based on the contribution, (iv) to exploit all subsidiary rights in the contribution, (v) the inclusion of electronic links from the contribution to third party material where-ever it may be located, and (vi) license any thrid party to do any or all of the above.