Antioxidant Activity and Cytotoxicity of the Leaf and Bark Extracts of Tarchonanthus camphorates
Purpose: To investigate the antioxidant potential and cytotoxicity of the leaf and bark extracts of Tarchonanathus campharatus..
Methods: The antioxidant activity of the aqueous leaf extract (Aq LF), methanol leaf extract (MET LF), dichloromethane leaf extract (DCM LF), methanol bark extract (MET BK), dichloromethane bark extract (DCM BK), and ethyl acetate bark extract (Et Ac BK) were examined by 1,1-Diphenyl-2-picryl-hydrazil (DPPH), 2f-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS.+), nitric oxide radical scavenging, reducing power and iron chelating activity methods. Brine shrimp lethality and MTT cytotoxicity tests
were used to investigate the cytotoxicity of the extracts.
Results: The Aq LF, DCM LF, MET LF and MET BK showed good DPPH, ABTS radical scavenging and total reducing power activities. Total reducing power was high and in the rank order of DCM LF > Aq LF > MET LF > MET BK. All the extracts, however, showed weak nitric oxide scavenging activity as
well as weak iron chelating ability. Flavonoids, phenols, tannins and saponins were present in some of the extracts, but alkaloids, terpenoids, cardenolides and cardiac glycosides were absent in all the extracts. All the extracts did not show significant cytotoxic properties (p . 0.05) with 50% inhibitory concentration IC50 values > 30 ƒÊg/ml in both cytotoxicity assays.
Conclusion: The antioxidant activity and low cytotoxicity of Tarchonanthus camphoratus probably justify its use in folk medicine.
Keywords: Tarchonanthus camphoratus, Antioxidant activity, Cytotoxicity
Submission of a manuscript to this journal is a representation that the manuscript has not been published previously and is not under consideration for publication elsewhere.
All authors named in each manuscript would be required to sign a form (to be supplied by the Editor) so that they may retain their copyright in the article but to assign to us (the Publishers) and its licensees in perpetuity, in all forms, formats and media (whether known or created in the future) to (i) publish, reproduce, distribute, display and store the contribution, (ii) translate the contribution into other languages, create adaptations, reprints, include within collections and create summaries, extracts and/or abstracts of the contribution, (iii) create any other derivative works(s) based on the contribution, (iv) to exploit all subsidiary rights in the contribution, (v) the inclusion of electronic links from the contribution to third party material where-ever it may be located, and (vi) license any thrid party to do any or all of the above.