Inhibitory Effect of Polysaccharides from Scutellaria barbata D. Don on Invasion and Metastasis of 95-D Cells Lines via Regulation of C-MET and E-CAD Expressions

  • XK Yang
  • YD Yang
  • SQ Tang
  • L XU
  • GH Yang
  • QY Xu
  • H Tang
  • JJ Wu
Keywords: Polysaccharide, Scutellaria barbata, 95-D cell lines, Invasion, Metastasis

Abstract

Purpose: To investigate the inhibitory effect of polysaccharides from Scutellaria barbata (PSB) on invasion and metastasis of lung cancer, and study the possible mechanism.
Methods: PSB was extracted with water and by alcohol precipitation, and purified by DEAE-52 column chromatography. A highly invasive and metastatic lung carcinoma cell, 95-D cell line, was used for the study. Cell adhesion and invasion assays in vitro were performed to evaluate the anti-invasive and antimetastatic effects of PSB (50 - 200 μg/ml) on 95-D cell. Immunocytochemical staining and Western blot techniques were employed to study the regulatory effects of PSB on the expression of C-MET and ECAD.
Results: The results indicate that PSB significantly inhibited cell invasion and migration of 95-D in a concentration-dependent manner (p < 0.05). The adhesion of 95-D cells to fibronectin was also inhibited by PSB (p < 0.05). The expression of C-MET and E-CAD in 95-D cells treated with PSB were significantly down-regulated and up-regulated, respectivelt (p < 0.05).
Conclusion: Treatment with PSB can significantly inhibit the invasion and metastasis of 95-D cells in vitro, probably through the regulation of C-MET and E-CAD.

Keywords: Polysaccharide, Scutellaria barbata, 95-D cell lines, Invasion, Metastasis

Published
2013-08-28
Section
Articles

Journal Identifiers


eISSN: 1596-9827
print ISSN: 1596-5996