PROMOTING ACCESS TO AFRICAN RESEARCH

Tropical Journal of Pharmaceutical Research

Log in or Register to get access to full text downloads.

Remember me or Register



Antiglycation and Antioxidant Activities and HPTLC Analysis of Boswellia sacra Oleogum Resin: The Sacred Frankincense

A Al-Harrasi, L Ali, E Ceniviva, A Al-Rawahi, J Hussain, H Hussain, NU Rehman, G Abbas, R Al-Harrasi

Abstract


Purpose: To evaluate antiglycation and antioxidant activities as undertake HPTLC analysis of Boswellia sacra resin.
Methods: Sub-fractionation of the crude methanol extract of Hougari regular (HR) grade resin of Boswellia sacra was carried out by vacuum liquid chromatography. Free radical scavenging and antiglycation activities of sub-fractions were characterized in order to assess their anti-aging properties. Furthermore, high performance thin layer chromatography (HPTLC) analysis of Boswellia sacra resins was also carried out.
Results: Polar fractions of the extract obtained exhibited the highest antiglycation activity while nonpolar fractions showed more than 50 % inhibition in superoxide anion scavenging assay. Scavenging activity of reactive oxygen species results indicate that non-polar sub-fractions showed > 50 % inhibition, except Shabi frankincense (SF) oil which showed 33 % inhibition. Dichloromethane (CH2Cl2) fraction, 40 % dichloromethane (CH2Cl2)/n-hexane sub-fraction, and SF oil showed moderate activity in di(phenyl)-(2,4,6-trinitrophenyl)iminoazanium (DPPH) assay. Furthermore, HPTLC analysis indicates the presence of 11-keto-β-boswellic acid (KBA) and 3-O-acetyl-11-keto-β-boswellic acid (AKBA) along with some other terpenoids.
Conclusion: Various sub-fractions of Boswellia sacra exert effective antiglycation and antioxidant activities. The extracts should be studied further for possible formulation into pharmaceutical products.

Keywords: Frankincense, Boswellia sacra, Terpenoids, HPTLC, Antioxidant, Antiglycation




http://dx.doi.org/10.4314/tjpr.v12i4.23
AJOL African Journals Online