PROMOTING ACCESS TO AFRICAN RESEARCH

Tropical Journal of Pharmaceutical Research

Log in or Register to get access to full text downloads.

Remember me or Register



Production of Methioninase from Serratia Marcescens Isolated from Soil and its Anti-Cancer Activity against Dalton’s Lymphoma Ascitic (DLA) and Ehrlich Ascitic Carcinoma (EAC) in Swiss Albino Mice

WA Sundar, H Nellaiah

Abstract


Purpose: To evaluate the anticancer activity of an enzyme, methioninase, obtained from a soil isolate, Serratia marcesens, against Dalton’s lymphoma ascitic (DLA) and Ehrlich ascitic carcinoma (EAC) in Swiss albino mice.
Methods: Cancer was induced using DLA and EAC cells in Swiss albino mice by intraperitoneal injection. The mice were then treated with 10 and 20 mg/kg body weight of methioninase; 5-fluorouracil (5-FU) was used as standard.
Results: The results showed good improvement in haematological parameters, improvement serum enzymes and lipid profile, as well as increase in life span by up to 77 %, increase in body weight (p < 0.01), decrease in cancer cell count from (2.70 ± 0.40) × 106 to (1.86 ± 0.30) × 106 cells/ml compared to (2.70 ± 0.40) × 106 to (1.42 ± 0.25) x 106 cells/ml for the group treated with 5-FU. Solid tumor volume decreased from 5.82 ± 0.15 to 4.10 ± 0.11 ml as as against a decrease from 5.82 ± 0.40 to 3.56 ± 0.28 for the group treated with 5-FU.
Conclusion: The isolated enzyme, methioninase, has a good anti-cancer activity and needs to be further be investigated for this activity.

Keywords: Methioninase, Anticancer, Serum depletion, Tumor, Dalton’s lymphoma ascetic, Ehrlich ascitic carcinoma




http://dx.doi.org/10.4314/tjpr.v12i5.6
AJOL African Journals Online