Enhancing the Production of a Novel Exopolysaccharide by Bacillus mucilaginosus CGMCC5766 Using Statistical Experiment Design
Purpose: To improve the production of a novel exopolysaccharide (EPS) by Bacillus mucilaginosus CGMCC5766.
Methods: The culture medium for production of EPS was optimized using statistical experiment design. Sucrose, CaCO3 and K2HPO4 were found to be the key factors based on the results obtained from Plackett–Burman experimental design. The optimal concentration range of the three factors was examined by the steepest ascent path, and then the optimum concentration was further investigated through response surface methodology. Validation experiments were also carried out to establish the adequacy and accuracy of the model obtained.
Results: Under optimal conditions (sucrose 61.31 g/l, CaCO3 5.06 g/l and K2HPO4 0.17 g/l), EPS concentration achieved 22.04 g/l, which was about 1.6-fold increase compared with that using the original medium. EPS concentration in 5-l fermenter reached 23.46 ± 0.76 g/l.
Conclusion: The optimization medium is considered fundamental and useful for the development of B. mucilaginosus CGMCC5766 cultivation process for efficient production of EPS on a large scale.
Keywords: Bacillus mucilaginosus CGMCC5766, Exopolysaccharide, Medium, Optimization, Statistical experiment design