Tropical Journal of Pharmaceutical Research

Log in or Register to get access to full text downloads.

Remember me or Register

Inhibitory Effect of the root of Polygala tenuifolia on Bradykinin and COX 2-Mediated Pain and Inflammatory Activity

JJ Oh, SJ Kim


Purpose: To gain insight into the mechanisms of analgesic and anti-inflammatory activities of the root extract of Polygala tenuifolia.
Methods: Polygala tenuifolia was extracted with 70 % methanol and tested for analgesic and antiinflammatory activities (0.1, 1, 10 and 100 mg/kg) using the following models: acetic acid-induced writhing, rat paw edema, bradykinin inhibtion with rat ileum, and prostaglandin assay.
Results: Administration of the Polygala tenuifolia extract at 100 mg/kg dose produced significant analgesic effect on acetic acid-induced writhing (97 % inhibition) but its effect in the tail-flick test was not significant (p < 0.05). In addition, the extract exerted significant anti-inflammatory effect in the rat paw edema model (8 to 33 % inhibition) at doses ranging from 0.1 - 100.0 mg/kg). A significant inhibitory action (53%) on the bradykinin-mediated contractions of rat ileum was also observed. Furthermore, the extract significantly (p < 0.05) inhibited the production of lipopolysaccharides-induced 6-keto-PGF by 28% in macrophage cultures.
Conclusion: These results provide evidence that the Polygala tenuifolia root extract exerts analgesic and anti-inflammatory effects via its significant inhibitory effect on acetic acid writhing test, bradykinin-mediated actions as well as on 6-keto-PGF induction.

Keywords: Polygalae radix, Bradylinin, Prostaglandin, COX-2, Inflammation, Analgesic
AJOL African Journals Online