Antibiogram and plasmid profiling of carbapenemase and extended spectrum Beta-lactamase (ESBL) producing Escherichia coli and Klebsiella pneumoniae in Abeokuta, South western, Nigeria

  • BO Motayo
  • PA Akinduti
  • FA Adeyakinu
  • PO Okerentugba
  • JC Nwanze
  • CC Onoh
  • HC Innocent-Adiele
  • IO Okonko

Abstract

Background: The increased reports of ESBL dissemination from various centres in south western, Nigeria and the recent emergence of carbapenem resistant bacteria prompted the conception of this study.
Objectives: To demonstrate the relationship between high molecular weight plasmids and the expression of antibiotic multi-resistance including ESBL and carbapenemase.
Methods: We investigated 97 isolates of selected organisms consisting of 67 E. coli and 30 Klebseilla spp for the presence of plasmids expressing ESBL including carbapenem-hydrolysing enzymes. Beta-lactamase was determined using acidometric method, while ESBL and carbapenemase activity was determined using the double-disk diffusion test as well as the Modified Hodge test (MHT). Plasmid profiles of ESBL and carbapenemase positive isolates were determined according to standard protocols.
Results: An ESBL prevalence rate of 21.6% and carbapenem- resistance rate of 9.3% was recorded. Antibiotic susceptibility profile of ESBL isolates showed 100.0% resistance against Amoxicillin, Cotrimoxazole and Erythromycin. Moderate susceptibility was recorded against the Quinolone class of antibiotics; Meropenem remained the most active antibiotic against ESBL isolates with 62.5% against E. coli and 60% against K. pneumoniae. The plasmid profiles of our study isolates ranged from 11.8kbp to 35.5kbp. Conclusion: Due to the relationship between high molecular weight plasmids and multi-drug resistance, we hereby recommend regular molecular surveillance of this form in our study setting.

Keywords: Carbapenem-resistance, ESBL isolates, Plasmid profile, Abeokuta

African Health Sciences 2013; 13(4): 1091 - 1097

Author Biographies

BO Motayo
Microbiology Unit, Pathology Department, Federal Medical Center, Abeokuta, Nigeria
PA Akinduti
Microbiology Unit, Pathology Department, Federal Medical Center, Abeokuta, Nigeria
FA Adeyakinu
Microbiology Unit, Pathology Department, Federal Medical Center, Abeokuta, Nigeria
PO Okerentugba
Medical Microbiology Unit, Department of Microbiology, University of Port Harcourt, East-West Road, P.M.B. 5323, Choba, Port Harcourt, Rivers State, Nigeria
JC Nwanze
Department of Pharmacology and Therapeutics, Igbinedion University, Okada, Edo State, Nigeria
CC Onoh
Department of Pharmacology and Therapeutics, Igbinedion University, Okada, Edo State, Nigeria
HC Innocent-Adiele
Medical Microbiology Unit, Department of Microbiology, University of Port Harcourt, East-West Road, P.M.B. 5323, Choba, Port Harcourt, Rivers State, Nigeria
IO Okonko
Medical Microbiology Unit, Department of Microbiology, University of Port Harcourt, East-West Road, P.M.B. 5323, Choba, Port Harcourt, Rivers State, Nigeria
Published
2014-02-02
Section
Articles

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eISSN: 1680-6905