Article Sidebar
Published:
Apr 8, 2024Keywords:
Article Details
While African Health Sciences has been freely accessible online there have been questions on whether it is Open Access or not. We wish to clearly state that indeed African Health Sciences is Open Access. There are key issues regarding Open Access needing clarification for avoidance of doubt:
- 1. Henceforth, papers in African Health Sciences will be published under the CC BY (Creative Commons Attribution License) 4.0 International. See details on https://creativecomons.org/)
- 2. The copyright owners or the authors grant the 3rd party (perpetually and in advance) the right to disseminate, reproduce, or use the research papers in part or in full, format/medium as long as:
- No substantive errors are introduced in the process
- Attribution of authorship and correct citation details are given
- The referencing details are not changed.
Should the papers be reproduced in part, this must be clearly stated.
- 3. The papers will be freely and universally accessible online in an easily readable format such as XML in at least one widely recognized open access repository such as PUBMED CENTRAL.
B. ABRIDGED LICENCE AGREEMENT BETWEEN AUTHORS AND African Health Sciences
I submitted my manuscript to African Health Sciences and would like to affirm that:
1.0 I am authorized by my co-authors to enter into these arrangements.
2.0 I guarantee, on behalf of self and co-authors:
- That the paper is original, and has not been published in any other peer-reviewed journal; nor is it under consideration by other journal (s). It does not infringe existing copyright or any other person’s rights
- That we are/I am the sole author(s) of the paper and with authority to enter into this agreement. My granting rights to African Health Sciences is not in breach of any other obligation
- That the paper contains nothing unlawful, or libelous. Nor anything that would constitute a breach of contract, confidence or commitment given to secrecy, if published
- That I/we have taken care to ensure the integrity of the article.
3.0 I and all co-authors, agree that the paper, if accepted for publication, shall be licensed under the Creative Commons Attribution License 4.0. (see https://creativecommons.org/)
Main Article Content
The cytotoxic, genotoxic and mitotoxic effects of Atractylis gummifera extract in vitro
Abstract
Background: The Mediterranean thistle Atractylis gummifera L. (Asteraceae; AG) has diterpenoid glucosides; atractyloside and
carboxyatractyloside that interact with mitochondrial protein adenine nucleotide translocator (ANT) and resulted in ATP inhibition.
Despite its well-known toxicity, acute poisonings still occur with this plant. Although most symptoms are attributed to
ANT and diterpenoids interaction, in-depth investigation of the effects of AG extract on various cellular processes has not been
performed.
Objective/method: We tested in vitro induction of mitochondrial permeability transition pore (MPTP) opening in bovine liver
mitochondria and evaluated its cytotoxicity and genotoxicity using Allium cepa test. Cell division, mitotic index (MI) and total
chromosomal and mitotic aberrations (TAs), that all seem potentially affected by ATP shortage, were studied in root cells of
Allium cepa exposed to Atractylis gummifera extract.
Results: With the two different doses of two purified AG fractions, stronger induction of MPTP was observed compared to
the induction with the standard pure atracyloside. Aqueous AG extract exerted inhibition root growth in A. cepa at 6 different
doses. The TAs was increased in a dose-dependent manner too, while mitotic index was decreased at the same doses. Evaluation
of mitotic phases revealed mitodepressive effect of AG on A. cepa roots.
Conclusion: This work highlights cellular and mitochondrial adverse effects of Atractylis gummifera extracts. A purified fraction
that likely corresponds to ATR derivatives induces MPTP opening leading to swelling of mitochondria and its dysfunction. Allium
cepa test provides the evidence for A. gummifera genotoxicity and cytotoxicity.
Keywords: Atractylis gummifera; Allium cepa test; mitochondrial permeability transition pore; genotoxicity; cytotoxicity; chromosomal
aberration; mitotic index.
