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African Journal of Biotechnology

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Comparison of ompP5 sequence-based typing and pulsed-filed gel electrophoresis for genotyping of Haemophilus parasuis

B Zhang, C Xu, J Zhang, L Zhang, L Guo, S Feng, M Liao

Abstract


In this study, comparison of the outer membrane protein P5 gene (ompP5) sequence-based typing with pulsed-field gel electrophoresis (PFGE) for the genotyping of Haemophilus parasuis, the 15 serovar reference strains and 43 isolates were investigated. When comparing the two methods, 31 ompP5 sequence types (STs) and 43 PFGE types were identified with ompP5 STs A16 and A28 being the dominant ompP5 ST types, while no predominant PFGE type was found. The discriminatory indices were 0.95 for ompP5 sequence-based method and 0.98 for PFGE, and the two techniques were proved to be 70.7% congruent. Therefore, ompP5 sequence-based typing was a simple and inexpensive method, enabling it as a preliminary technique to research the molecular differentiation of H. parasuis.

Key words: Haemophilus parasuis, ompP5-based typing method, PFGE, molecular typing.




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