Assessment of Phaseolus vulgaris L and Vigna unguiculata (L.) Walp leaves for antifungal metabolites against two bean fungal pathogens Colletotricum lindemuthianum and Phaeoisariopsis griseola

  • Diana Alexandra Norena-Ramirez
  • Oscar Julian Velasguez-Ballesteros
  • Elizabeth Murillo-Perea
  • John Mendez-Arteaga
  • Joze Fernando Solanilla-Duque
  • Walter Murillo-Arango

Abstract

The antifungal potential of hydro-ethanolic leaf extracts of bean varieties was analyzed by bioautography, assessing the contribution of defense molecules of proteic nature against two bean fungal pathogens Colletotricum lindemuthianum and Phaeoisariopsis griseola, also, the Rf values and relative activities of separated compounds were determined; these compounds were in a range of medium polarity to high polarity. The bioactivity expressed by the studied bean varieties could be correlated with the presence of proteic nature compounds, in joint action with secondary plant metabolites. There was some similarity in the chemical composition of the components of the extracts. The varieties G18350, G14241, G1320 CIAT and Vigna ungiculata were the most promising for isolating antifungal compounds. The results demonstrate the value of bioautography as a simple and cheap method to examine plant extracts with antifungal activity.

Keywords: Phaseolus vulgaris LVigna unguiculata (L.) Walp, bioautography, peptides, secondary metabolites, Colletotricum lindemuthianum, Phaeoisariopsis griseola, antifungal defense

African Journal of Biotechnology, Vol 13(15), 1657-1665

Author Biographies

Diana Alexandra Norena-Ramirez
Investigation group, Chemistry of Natural Products, Department of Chemistry, Science Faculty, University of Tolima, Ibague, Colombia
Oscar Julian Velasguez-Ballesteros
Investigation group, Chemistry of Natural Products, Department of Chemistry, Science Faculty, University of Tolima, Ibague, Colombia
Elizabeth Murillo-Perea
Investigation group, Chemistry of Natural Products, Department of Chemistry, Science Faculty, University of Tolima, Ibague, Colombia
John Mendez-Arteaga
Investigation group, Chemistry of Natural Products, Department of Chemistry, Science Faculty, University of Tolima, Ibague, Colombia
Joze Fernando Solanilla-Duque
Investigation group, GEDAGRITOL, Department of production and plant health, Agronomy facult, University of Tolima, Ibaque, Colombia
Walter Murillo-Arango
Investigation group, Chemistry of Natural Products, Department of Chemistry, Science Faculty, University of Tolima, Ibague, Colombia
Published
2015-06-29
Section
Articles

Journal Identifiers


eISSN: 1684-5315