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African Journal of Biotechnology

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Analysis of genetic variability in rice cultivars of Arunachal Pradesh (India) using microsatellite marker

SR Berilus, A Pattanayak, Gurudayal Ram

Abstract


The present investigation was carried out to analyze the genetic diversity of rice cultivars utilizing microsatellite or simple sequence repeat (SSR) markers. Twenty four (24) genotypes of rice were evaluated for polymorphisms after amplification with 30 SSR primer pairs. The results obtained depicted a wide range of variation among the varieties. The diversity parameters confirmed that Bordungsha F1, S5 and Baodhan Bordungsha (deep water rice) were most diverse and were distinctly different from the rests of the genotype and the dissimilarity coefficient ranged from 0.06 to 0.94. This study provides a clear picture of the genetic diversity of rice. Among the set of SSR primers tested, RM44 (0.942), RM495 (0.932), RM125 (0.998) and OSR13 (0.970) were more informative in comparison to other primers.

Keywords: Oryza sativa, SSR markers, PCR, polymorphism, genetic variation

African Journal of Biotechnology Vol. 12(8), pp. 798-810



AJOL African Journals Online