Enzymatic biodiesel production from sludge palm oil (SPO) using locally produced Candida cylindracea lipase

  • Ricca Rahman Nasaruddin
  • Md. Zahangir Alam
  • Mohammed Saedi Jami

Abstract

Biodiesel is a non-toxic, renewable and environmental friendly fuel. This study involved the production of biodiesel from sludge palm oil (SPO), a low-cost waste oil via enzymatic catalysis. The enzyme catalyst was a Candida cylindracea lipase, locally-produced using palm oil mill effluent as the low cost based medium. The results in solvent system for biodiesel production showed that ethanol gave higher yield of biodiesel as compared to methanol. One-factor-at-a time (OFAT) method was applied to investigate several factors for enzymatic biodiesel production. The optimum levels of ethanol-to-SPO molar ratio, enzyme loading, reaction temperature, mixing speed and reaction time were 4:1, 10 U, 40°C, 250 rpm and 24 h, respectively with maximum yield of biodiesel of 62.3% (w/w SPO). The SPO had a promising potential for enzymatic biodiesel production using locally-produced lipase.

Keywords: Biodiesel, sludge palm oil, lipase, free fatty acid, fatty acid alkyl ester

African Journal of Biotechnology Vol. 12(31), pp. 4966-4974

Author Biographies

Ricca Rahman Nasaruddin
Bioenvironmental Engineering Research Centre (BERC), Department of Biotechnology Engineering, Faculty of Engineering International Islamic University Malaysia, P.O. Box 10, 50728 Kuala Lumpur, Malaysia
Md. Zahangir Alam
Bioenvironmental Engineering Research Centre (BERC), Department of Biotechnology Engineering, Faculty of Engineering International Islamic University Malaysia, P.O. Box 10, 50728 Kuala Lumpur, Malaysia
Mohammed Saedi Jami
Bioenvironmental Engineering Research Centre (BERC), Department of Biotechnology Engineering, Faculty of Engineering International Islamic University Malaysia, P.O. Box 10, 50728 Kuala Lumpur, Malaysia
Published
2016-04-18
Section
Articles

Journal Identifiers


eISSN: 1684-5315