Jozala, A. F., de Lencastre Novaes, L. C., Cholewa, O., & Vessoni Penna, T. C. (2005). Increase of nisin production by Lactococcus lactis in different media. African Journal of Biotechnology, 4(3), 262–265. https://doi.org/10.4314/ajb.v4i3.15092
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Main Article Content
Increase of nisin production by Lactococcus lactis in different media
Angela Faustino Jozala
Department of Biochemical and Pharmaceutical Technology School of Pharmaceutical Sciences, University of São Paulo /SP, Brasil
Letícia Célia de Lencastre Novaes
Department of Biochemical and Pharmaceutical Technology School of Pharmaceutical Sciences, University of São Paulo /SP, Brasil
Olivia Cholewa
Molecular Probes, Inc., Eugene, Or, USA. 97402
Thereza Christina Vessoni Penna
Department of Biochemical and Pharmaceutical Technology School of Pharmaceutical Sciences, University of São Paulo /SP, Brasil
Abstract
Nisin production related to the growth conditions of Lactococcus lactis subsp. lactis ATCC 11454, the effects of various media components and concomitant release of nisin into the media, were studied through transfers (five times). Nisin production was assayed by agar diffusion using Lactobacillus sake ATCC 15521 as the sensitive test organism. The expression of nisin was strongly influenced by the addition of skimmed milk to both MRS and M17 broth, with the highest production obtained after the second and the fifth transfers, respectively, with maximum expression after 36 h of incubation.
African Journal of Biotechnology Vol. 4 (3), pp. 262-265, 2005
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