Volumetric and viscometric behaviour of soya bean and gram proteins in aqueous methotrexate (anticancer drug) solution at 298.15 to 308.15K
AbstractApparent molar volumes (Vf) and viscosities (h) for 0.00005 to 0.0004 kg.mol-1 (50 to 400 mmol kg-1) aqueous methotrexate (MTX), gram (Gp) and soya bean (SBp) proteins, and similar compositions of Gp
and SBp each in 50 to 400 mmol kg-1 aqueous MTX were determined at 298.15, 303.15 and 308.15K. The Vf values are positive except aqueous MTX and listed as SBp > Gp > MTX in aqueous and SBp > Gp in MTX solutions, respectively. It infers weaker hydrophobic heteromolecular interaction of SBp in binary and ternary systems. The higher h values of SBp and Gp in MTX than those of aqueous MTX prove strengthening of hydrophobic interactions of proteins by MTX. It illustrates the conformational changes of proteins; slightly higher Vf values of SBp than of Gp. The MTX confirm higher structural activity in biological process. The Vf 0 of MTX increases with K and decrease with composition. But with compositions the values continuously decrease with lower magnitude.