Protective antitumor activity induced by a fusion vaccine with murine beta-defensin2 and VE-cadherin in mouse models

  • J Xu
  • L Wang
  • J Zhao
  • G Wang
  • G Xie
  • Y Wu
  • H Li
  • X Du
  • P Diao
  • H Yang
  • Y Wen
  • R Wang
  • H Wu
  • Y Wei
  • Y Wang
Keywords: Fusion vaccine, murine beta defensin2 (MBD2), murine vascular endothelial-cadherin (mVE-cad), antigen targeting, anti-angiogenesis.


Targeting angiogenesis is an effective strategy for anticancer therapy. The vascular endothelialcadherin (VE-cad) regulated angiogenesis is a potential target for anti-angiogenesis. Here, we develop a fusion vaccine plasmid DNA pSec-MBD2-VE-cad from VE-cad and murine beta defensin2 (MBD2) to induce immunity for cancer therapy. The expression and biological activity of fusion protein were detected


in vitro. Anti-tumor effects and inhibition of angiogenesis via pSec-MBD2-VE-cad were investigated in mice model. The anti-VE-cad antibodies and cytotoxic T lymphocyte (CTL) responses were analyzed. Inhibition of tumor-induced angiogenesis and prolonged survival were shown in mice challenged with murine colon adenocarcinoma (CT26) or Murine fibrosarcoma cell line (MethA) after immunization with the fusion vaccine. Moreover, VE-cad-specific antibodies and specific T cell cytotoxicity were detected. The fusion vaccine based on self immune peptide Murine beta defensin2 (MBD2) and self antigen mVE-cad could induce autoimmunity and inhibit tumor growth, and thus there may be potential applications in cancer therapy.


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eISSN: 1684-5315