Full Length Research Paper Curcumin induces cleavage of -catenin by activation of capases and downregulates the β-catenin/Tcf signaling pathway in HT-29 cells

  • LL Qi
  • JB Wang
  • HZ Wang
  • YT Luo
  • TX Wu
Keywords: Curcumin, &#946-catenin/Tcf signaling pathway, HT-29 cells.

Abstract

β-Catenin/Tcf-4 signaling pathway plays important roles in colorectal tumorigenesis. RT-PCR, western blotting and immunoprecipitation were used to study the effects of curcumin on β-catenin/Tcf-4 signaling pathway in HT-29 cells. Treatment of curcumin could induce cleavage of β-catenin and the cleavage could be inhibited by caspase inhibitors. The association of β-catenin with Tcf-4 in nucleus could be inhibited by curcumin. The expression of c-myc and cyclinD1 was downregulated by curcumin, which could not be blocked by Z-DEVD-FMK. The results showed curcumin could induce the
cleavage of β-catenin by activition of caspases and downregulate the activity of β-catenin/Tcf signaling pathway independent of the caspases in HT-29 cells.
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eISSN: 1684-5315