Cloning and expression of Phanerochaete chrysosporium cellobiohydrolase (cbhI.2)

  • RL Howard
Keywords: Phanerochaete chrysosporium, cbhI.2, cloning and expression, pulse-labelling

Abstract

The Phanerochaete chrysosporium ME446 cbhI.2 cDNA was cloned and expressed in Escherichia coli using a pET system. Pulse-labelling revealed the expression of an induced protein of approximately 50- 60 kDa that reacted with anti-P. chrysosporium ME446 CBH antibodies. The expressed protein remained undegraded in vivo for 135 min but was biologically inactive in vitro.
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eISSN: 1684-5315