PROMOTING ACCESS TO AFRICAN RESEARCH

African Journal of Biomedical Research

Log in or Register to get access to full text downloads.

Remember me or Register



Cardiospermum halicacabum suppresses the production of TNF-alpha and nitric oxide by human peripheral blood mononuclear cells

B.K.C Venkatesh, S Krishnakumari

Abstract


Cardiospermum halicacabum is an Asian and African herb known to
possess anti-inflammatory activities. The inhibitory effects of the ethanolic
fraction of Cardiospermum halicacabum leaves extract,(EFC) on the
production of pro-inflammatory mediators, nitric oxide(NO) and tumor
necrosis factor-alpha (TNF a) were studied in a lipopolysacchride (LPS)
activated human peripheral blood mononuclear cells. TNF a production
was measured based on the selective sensitivity of TNF a for L929
tumorigenic murine cells. Similarly LPS stimulated macrophages cell line
RAW 264.7 was used for NO inhibition studies. EFC potently inhibited
TNF a (5-40μg/mL) with IC50=17 μg/mL, and NO (20-100 μg/mL) with
IC50 value being 90μg/mL.The viability of cells at all the concentration
was unaffected as determined by the MTS cytotoxicity
assay.C.halicacabum thus exhibits the anti-inflammatory properties,
justifying its use in rheumatoid arthritis treatment. (Afr. J. Biomed. Res. 9:
95 -99, May 2006)

 

Keywords: Cardiospermum halicacabum, nitric oxide, TNF a.




http://dx.doi.org/10.4314/ajbr.v9i2.48781
AJOL African Journals Online