PROMOTING ACCESS TO AFRICAN RESEARCH

Alexandria Journal of Medicine

Log in or Register to get access to full text downloads.

Remember me or Register



A method optimization study for atomic absorption spectrophotometric determination of total zinc in insulin using direct aspiration technique

S Ata, FH Wattoo, M Ahmed, MHS Wattoo, SA Tirmizi, A Wadood

Abstract


A sensitive, reliable and relative fast method has been developed for the determination of
total zinc in insulin by atomic absorption spectrophotometer. This designed study was used to optimize the procedures for the existing methods. Spectrograms of both standard and sample solutions of zinc were recorded by measuring the absorbance at 213.9 nm for determination of total zinc. System suitability parameters were evaluated and were found to be within the limits. Linearity was evaluated through graphical representation of concentration versus absorbance. Repeatability (intra-day) and intermediate precision (inter-day) were assessed by analyzing working standard solutions. Accuracy and robustness were experimented from the standard procedures. The percentage recovery of zinc was found to be 99.8%, relative standard deviation RSD 1.13%, linearity of determination LOD 0.0032 lg/mL, and limit of quantization LOQ 0.0120 lg/mL. This developed and proposed method was then validated in terms of accuracy, precision, linearity and robustness which can be successfully used for the quantization of zinc in insulin.




http://dx.doi.org/10.1016/j.ajme.2014.03.004
AJOL African Journals Online